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Abstract BackgroundLongitudinal skeletal growth takes place in the cartilaginous growth plates. While growth plates are found at either end of conventional long bones, they occur at a variety of locations in the mammalian skeleton. For example, the metacarpals and metatarsals (MT) in the hands and feet form only a single growth plate at one end, and the pisiform in the wrist is the only carpal bone to contain a growth plate. We take advantage of this natural anatomical variation to test which components of the PTHrP/Ihh feedback loop, a fundamental regulator of chondrocyte differentiation, are specific to growth plate function. ResultsParathyroid hormone‐like hormone(Pthlh), the gene that transcribes parathyroid hormone‐related peptide (PTHrP), is expressed in the reserve zone of the growth plate‐forming end of the MT. At the opposite end, the absence of a PTHrP+ reserve zone results in premature chondrocyte differentiation andIndian hedgehog(Ihh) expression.Pthlhis expressed in the reserve zone of the developing pisiform, confirming the existence of a true growth plate. ConclusionA pool of PTHrP+ reserve zone chondrocytes is a defining characteristic of growth plates, and its patterning may be key to evolved differences in growth plate location in the mammalian skeleton. 

ABSTRACT We observed a novel, nocturnal cleaning interaction between a cleaner shrimp (GenusUrocaridella) and the giant moray eel (Gymnothorax javanicus) on a lagoonal patch reef in Moorea, French Polynesia. Over the course of an 85‐min foraging bout (recorded on video by a snorkeler), we observed three separate, stereotyped cleaning interactions betweenG. javanicusand a cleaner shrimp in the genus Urocaridella (which surveys of Moorea biodiversity previously visually identified asUrocaridella antonbruunii). During these interactions, the shrimp would slowly crawl along one of the eel's flanks towards its head, enter its mouth, emerge on the other side of its head, then crawl back towards the reef along the eel's opposite flank, often causing it to jolt in response. On each of the visits, the moray spent roughly 9–12 min at the cleaning station and was observed being cleaned for a total of 62 s. Although this was a chance observation of only a few instances of cleaning, it may have several important implications for our understanding of the behavioral ecology of cleaning mutualisms, including (1) indicating potential temporal trade‐offs between being cleaned and foraging in eels, (2) suggesting a degree of temporal niche partitioning among sympatric cleaner species and (3) updating our understanding of cleaner‐client communication, given the nocturnal nature of our observations. 

This work describes the effect of varying crosslink density and plasticizer loading on covalent adaptable networks that have equal amounts of reactive functionalities. 

Arizona is home to many mosquito species, some of which are known vectors of infectious diseases that harm both humans and animals. Here, we provide an overview of the 56 mosquito species that have been identified in the State to date, but also discuss their known feeding preference and the diseases they can (potentially) transmit to humans and animals. This list is unlikely to be complete for several reasons: (i) Arizona’s mosquitoes are not systematically surveyed in many areas, (ii) surveillance efforts often target specific species of interest, and (iii) doubts have been raised by one or more scientists about the accuracy of some collection records, which has been noted in this article. There needs to be an integrated and multifaceted surveillance approach that involves entomologists and epidemiologists, but also social scientists, wildlife ecologists, ornithologists, representatives from the agricultural department, and irrigation and drainage districts. This will allow public health officials to (i) monitor changes in current mosquito species diversity and abundance, (ii) monitor the introduction of new or invasive species, (iii) identify locations or specific populations that are more at risk for mosquito-borne diseases, and (iv) effectively guide vector control. 

A new framework, called , for the combined study of both hard and soft transverse momentum sectors in high-energy proton-proton ( $p\text{−}p$) and proton-nucleus ( $p\text{−}A$) collisions is set up. A dynamical initial state is set up using the model with transverse locations of hotspots within each incoming nucleon. A hard scattering that emanates from two colliding hotspots is carried out using the Pythia generator. Initial state radiation from the incoming hard partons is carried out in a new module called , which includes the longitudinal location of initial splits. The energy-momentum of both the initial hard partons and their associated beam remnants is removed from the hot spots, depleting the energy-momentum available for the formation of the bulk medium. Outgoing showers are simulated using the generator, and results are presented for both cases, allowing for and not allowing for energy loss. First comparisons between this hard-soft model and single inclusive hadron and jet data from $p\text{−}p$and minimum bias $p\text{−}\text{Pb}$collisions are presented. Single hadron spectra in $p\text{−}p$are used to carry out a limited (in number of parameters) Bayesian calibration of the model. Fair comparisons with data are indicative of the utility of this new framework. Theoretical studies of the correlation between jet $p_T$and event activity at mid and forward rapidity are carried out. 

ABSTRACT Marine macroalgae produce abundant and diverse polysaccharides, which contribute substantially to the organic matter exported to the deep ocean. Microbial degradation of these polysaccharides plays an important role in the turnover of macroalgal biomass. Various members of thePlanctomycetes-Verrucomicrobia-Chlamydia(PVC) superphylum are degraders of polysaccharides in widespread anoxic environments. In this study, we isolated a novel anaerobic bacterial strain NLcol2^Tfrom microbial mats on the surface of marine sediments offshore Santa Barbara, CA, USA. Based on 16S ribosomal RNA (rRNA) gene and phylogenomic analyses, strain NLcol2^Trepresents a novel species within thePontiellagenus in theKiritimatiellotaphylum (within the PVC superphylum). Strain NLcol2^Tis able to utilize various monosaccharides, disaccharides, and macroalgal polysaccharides such as agar and ɩ-carrageenan. A near-complete genome also revealed an extensive metabolic capacity for anaerobic degradation of sulfated polysaccharides, as evidenced by 202 carbohydrate-active enzymes (CAZymes) and 165 sulfatases. Additionally, its ability of nitrogen fixation was confirmed by nitrogenase activity detected during growth on nitrogen-free medium, and the presence of nitrogenases (nifDKH) encoded in the genome. Based on the physiological and genomic analyses, this strain represents a new species of bacteria that may play an important role in the degradation of macroalgal polysaccharides and with relevance to the biogeochemical cycling of carbon, sulfur, and nitrogen in marine environments. Strain NLcol2^T(= DSM 113125^T= MCCC 1K08672^T) is proposed to be the type strain of a novel species in thePontiellagenus, and the namePontiella agarivoranssp. nov. is proposed.IMPORTANCEGrowth and intentional burial of marine macroalgae is being considered as a carbon dioxide reduction strategy but elicits concerns as to the fate and impacts of this macroalgal carbon in the ocean. Diverse heterotrophic microbial communities in the ocean specialize in these complex polymers such as carrageenan and fucoidan, for example, members of theKiritimatiellotaphylum. However, only four type strains within the phylum have been cultivated and characterized to date, and there is limited knowledge about the metabolic capabilities and functional roles of related organisms in the environment. The new isolate strain NLcol2^Texpands the known substrate range of this phylum and further reveals the ability to fix nitrogen during anaerobic growth on macroalgal polysaccharides, thereby informing the issue of macroalgal carbon disposal. 

The transition to motherhood in mammals is marked by changes in females’ perception of and responsiveness to sensory stimuli from infants. Our understanding of maternally induced sensory plasticity relies most heavily on studies in uniparental, promiscuous house mice and rats, which may not be representative of rodent species with different life histories. We exposed biparental, monogamous California mouse (Peromyscus californicus) mothers and ovariectomized virgin females to one of four acoustic and olfactory stimulus combinations (Control: clean cotton and white noise; Call: clean cotton and pup vocalizations; Odor: pup-scented cotton and white noise; Call + Odor: pup-scented cotton and pup vocalizations) and quantified females’ behavior and Fos expression in select brain regions. Behavior did not differ between mothers and ovariectomized virgins. Among mothers, however, those exposed to the Control condition took the longest to sniff the odor stimulus, and mothers exposed to the Odor condition were quicker to sniff the odor ball compared to those in the Call condition. Behavior did not differ among ovariectomized virgins exposed to the different conditions. Fos expression differed across conditions only in the anterior hypothalamic nucleus (AHN), which responds to aversive stimuli: among mothers, the Control condition elicited the highest AHN Fos and Call + Odor elicited the lowest. Among ovariectomized virgin fe- males, Call elicited the lowest Fos in the AHN. Thus, reproductive status in California mice alters females’ behavioral responses to stimuli from pups, especially odors, and results in the inhibition of defense circuitry in response to pup stimuli.